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KMID : 0379120150430040286
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Korean Journal of Mycology
2015 Volume.43 No. 4 p.286 ~ p.289
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Optimal Storage Condition of Clubroot Pathogen, Plasmodiophora brassicae for Artificial Inoculation
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Yang Seul-Gi
Park Ju-Young
Seo Moon-Won
Kim Hong-GI
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Abstract
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Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is a severe soilborne disease of Brassicaceae. Storage of clubroot gall is important for studies on pathogenicity and race identification. As the current storage method has been used for more than 100 years, a new storage method should be developed and the most efficient way maintaining pathogenicity should be determined. Effects of storage conditions with different storage periods on pathogenicity in galls of kimchi cabbage were examined in a greenhouse. The experiments were performed under six conditions and four temperatures in order to determine the most effective storage conditions for maintenance of pathogenicity. The most effective conditions for clubroot gall storage was the storage of whole gall at -70oC or storage of filtrate at the same temperature through eight layers of gauze after homogenization of the galls.
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KEYWORD
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Clubroot gall, Optimal storage condition, Pathogenicity, Plasmodiophora brassicae, -70oC
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